Facilities and Equipment
What types of facilities and equipment are used for SC-INBRE research?
The three-story 66,000 sq. ft. science building was constructed in 1991. The Department of Biology occupies over 75% of Dalton Hall. The building contains 1,200 sq. ft. laboratories for each faculty member. In addition, there is a 2,400 sq. ft. large equipment room, autoclave facility, 1,000 sq. ft. cell culture lab, 2,000 sq. ft. optical and electron microscopy suite, and 1,500 sq. ft. computer lab available for research. INBRE research equipment and tissue culture facilities are maintained by Dr. Kim Wilson, Biomedical Research Manager. Dr. Julian Smith III, Director of the Microscopy, manages the microscopy suite and often shares his expertise with many of the INBRE investigators and also collaborates on numerous INBRE projects.
The Biology Department houses only half of the Biomedical Core Research Laboratory. Additional equipment is housed in the Department of Chemistry in the recently renovated 60,000 sq. ft. Sims Science Building.
Large Equipment Room Facility
- Applied Biosystems 7300 Real-time PCR machine
RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation. Real-time PCR differs from traditional RT-PCR because it allows one to actually view the increase in the amount of DNA as it is amplified using a fluorescent amplicon. Real-time PCR is by far the best method for quantitating changes in gene expression.
- Bio-tek FLx800 Microplate Fluorescence Reader
Fluorescence detection is a widely used method for the conducting endpoint, kinetic, flash, area scanning assays; DNA quantification; protein quantificaion; ion channel assays, enzyme activity, gene expression (reporter gene assays); cell-based assays; and FRET assays.
- ChemiImager™ 4000 by Alpha Innotech
The Chemilmager™ 400 Imaging System is ideal for detecting fluorescence such as DNA stained with edithium bromide and western blots stained with luminol. The system uses a Charged Coupling Device (CCD) camera at 40°C.
- Leica CM 1850 Cryostat
The Leica CM 1850 contains a microtome that is used to cut sections of tissue from 5 - 30 µm. The sections can then be fixed and stained with antibodies to look at specific molecules in the samples.
- Licor 4300 DNA Analysis System
The Licor 4300 DNA Analysis System is a basic DNA sequencing system. The DNA sequences of plasmid DNA or polymerase chain reaction (PCR) products can be determined using fluorescent primers that are specific to the template. Samples are loaded on to a polyacrylamide gel and an electric current is applied. The current causes the sample to migrate down the gel. Sequences are then determined using e-Seq™ Software.
- Sorvall Evolution and Discovery Centrifuges
The Sorvall Evolution and the Sorvall Discovery centrifuges are used for high speed centrifugation of samples. The Evolution is a super speed refrigerated unit that can hold samples from 50 ml to 1 liter and reach speeds up to 50,000g. The Discovery is an ultracentrifuge that holds micro-sized samples ranging from 200 - 1500 µl and can reach speeds up to 100,000 rpm.
- Sorvall Legend RT Centrifuge
The Sorvall Legend RT is a refrigerated micro-centrifuge that is equipped with a swinging rotor that holds 15 and 50 ml tubes. The centrifuge can reach speeds form 300 - 15,000 rpm depending on the rotor. This centrifuge is routinely used to pellet tissue culture cells.
- ChemWell 96
- Kendro and Thermo-Forma CO2 incubators
- LKG Densitometer
- Olympus Inverted Microscope with Light Box
Core Molecular Biology Facility
The core molecular biology facility is currently undergoing renovations in Dalton Hall. Many of the equipment for the facility has already been purchased. The facility will be equipped with a real-time PCR machine, several thermocyclers, an RT-PCR hood, an RNA workbench, waterbaths, heatblocks, electrophoresis equipment, and several other minor equipment items for performing routine molecular biology applications.
Tissue Culture Facility
In addition, Dalton Hall possesses a major microscopy suite that includes a Zeiss Transmission Electron Microscope (TEM), ISI Scanning electron Microscope (SEM), a Molecular Dynamics MD 2001Multiprobe confocal laser scanning microscope system, and two inverted light microscopes.